Analysis Note

ControlP19 mouse embryonic carcinoma cells

Application

Research Sub CategoryPluripotent & Early DifferentiationNeural Stem Cells

Research CategoryStem Cell Research

FACS

Immunocytochemistry:

1. Fix cells with a 4% Paraformaldehyde solution for 30-45 minutes, and then rinse several times with Tris-Buffered-Saline (TBS).

2. Incubate cells with MAB4301X diluted to 1/100 with PBS/ Tween-20 (0.05%) / BSA (1%) for 30 minutes.

3. Wash the sample three times with PBS / Tween-20 (0.05%) for 1 minute per wash. Examine under a microscope fitted with a FITC filter.

Optimal working dilutions must be determined by end user.

This Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488 is validated for use in FC, IC, IF for the detection of Stage-Specific Embryonic Antigen-1.

Disclaimer

Alexa Fluor^® is a registered trademark of Molecular Probes, Inc.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Immunogen

F9 teratocarcinoma stem cells (X-irradiated)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Ammonium Sulfate Precipitation

Purified immunoglobulin conjugated to Alexa Fluor^® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide

Specificity

Reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Storage and Stability

Maintain refrigerated at 2–8°C protected from light in undiluted aliquots for up to 24 months.